Exploring the chemodiversity of antimicrobial minalemines from Didemnum granulatum by neutral loss graph
In this study, we report on the identification of the active antimicrobial principles from the tunicate Didemnum granulatum. Two new natural products, minalemines G (1) and H (2), were isolated and their structures were elucidated. Both compounds showed potent antibacterial activity, with compound 1 showing sub-microgram inhibition against Staphylococcus aureus. Moreover, we also report a new mass spectrometry-based approach, named neutral loss graph, developed to explore minor metabolites from complex mixtures. This approach was applied here to reveal twelve additional minalemine analogues in the organic extract of D. granulatum.
Authors
- Jason R. Evans
- Rhone K. Akee
- Vítor F. Freire (ORCID: https://orcid.org/0000-0001-6565-1830)
- Lucero Martínez-Fructuoso (ORCID: https://orcid.org/0000-0002-2279-5479)
- Barry R. O’Keefe (ORCID: https://orcid.org/0000-0003-0772-4856)
- Christopher C. Thornburg (ORCID: https://orcid.org/0000-0002-4657-6895)
- Susan M. Ensel (ORCID: https://orcid.org/0000-0002-7733-1065)
- Dong Wang (ORCID: https://orcid.org/0000-0001-7877-7440)
- Rohitesh Kumar
- Tanja Grkovic
- Brian D. Peyser
- Svetlana Hogan
Institutions
- Leidos (United States) (US)
- Frederick National Laboratory for Cancer Research (US)
- National Cancer Institute (US)
- Center for Cancer Research (US)
- Hood College (US)
Publication Details
- Journal
- Scientific Reports
- Published
- 2026-01-07
- DOI
- https://doi.org/10.1038/s41598-025-32070-2
- Primary Topic
- Marine Sponges and Natural Products
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Health and Human Services
- National Institutes of Health
- National Cancer Institute
- National Institute of Allergy and Infectious Diseases