Association of CSF cortisol with brain glucose metabolism in Alzheimer's disease continuum

Abstract Background Elevated cortisol levels are considered a risk factor for Alzheimer's disease (AD). However, the relationship between cerebrospinal fluid (CSF) cortisol levels and AD pathophysiology remains insufficiently explored. Brain glucose hypometabolism, assessed with fluorodeoxyglucose positron emission tomography ([18F]FDG‐PET), is a hallmark of neurodegeneration in AD. Understanding the impact of CSF cortisol levels on brain metabolism is crucial for elucidating cortisol's role in the mechanisms underlying AD. This study aimed to investigate the association between CSF cortisol levels and [18F]FDG‐PET imaging across the biological and clinical continuum of AD. Method We analyzed 133 participants from ADNI with available CSF cortisol measures, [18F]FDG‐PET imaging, and CSF Elecsys biomarkers (Aβ1‐42 and p ‐tau181). Voxel‐wise linear regressions were conducted to evaluate the baseline associations between CSF cortisol levels and [18F]FDG‐PET using three models: (1) CSF cortisol as the independent variable; (2) interaction between CSF cortisol and clinical diagnoses (cognitive unimpaired and cognitive impaired); (3) interaction between CSF cortisol and AD biomarker positivity (Aβ1‐42 and p ‐tau181 positivity). Models were adjusted for age, gender, APOEɛ4 status, cognitive status, mini‐mental state exam (MMSE) and geriatric depression scale (GDS) scores, and Aβ1‐42 and p ‐tau181 levels. Results were corrected for multiple comparisons using random field theory (RFT, p < 0.001). Result Demographics are depicted in Table 1. Voxel‐wise analyses identified a significant association between elevated CSF cortisol levels and brain glucose hypometabolism in several AD‐related brain regions, comprising the parietal lobe, lateral fronto‐orbital gyrus, right precuneus, frontal lobe, and inferior frontal gyrus (t‐value = ‐3.1588; Figure 1). Furthermore, no interactions were observed between CSF cortisol levels and clinical diagnoses or AD biomarker positivity in relation to brain metabolism (data not shown). Conclusion This study demonstrated that CSF cortisol levels are associated with brain glucose hypometabolism in regions commonly vulnerable to AD. Notably, these associations were independent of AD biomarker positivity or clinical diagnoses. These findings suggest that elevated central cortisol levels may increase vulnerability in brain regions typically affected by AD, irrespective of the underlying pathophysiology. This underscores the potential role of cortisol as a risk‐related marker rather than a direct risk factor for AD, offering valuable insights into its contribution to disease mechanisms.