Metabolic engineering of Escherichia coli BW25113 for the production of Vitamin K2 based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification
The stepwise engineering strategy raised the VK<sub>2</sub> titer from the initial 303 mg/L to 859 mg/L through rational pathway modification and systematic gene expression. Further optimization in batch fermentation increased the VK<sub>2</sub> titer to 1360 mg/L, which highlights the strong engineering impact of our strategy.
Authors
- Changchuan Ye (ORCID: https://orcid.org/0000-0002-0683-5290)
- Yan Zhang (ORCID: https://orcid.org/0000-0002-9360-5388)
- Menglei Shi (ORCID: https://orcid.org/0009-0009-6570-5304)
- Feixue Nie
- Qinghua Liu
- Jie Zhang
Institutions
- Fujian Agriculture and Forestry University (CN)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2026-01-08
- DOI
- https://doi.org/10.1186/s13036-025-00614-9
- Primary Topic
- Vitamin K Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00