VERNONIA AMYGDALINA (BITTER LEAF) SHOWCASES RESTORATIVE POTENTIALS ON THE RENAL HISTOARCHITECTURE OF WISTAR RAT MODELS FOLLOWING ALLOXAN-INDUCED DIABETES MELLITUS

Abstract: Background: Diabetic patients’ are at risk of developing systemic complications such as nephropathy on the long run. Vernonia amygdalina (Bitter leaf) has clearly displayed anti-diabetic and antioxidant properties in several studies. Aim: This study was aimed at investigating effects of the MLEVA; methanol leaf extract of Vernonia amygdalina (bitter leaf) on the histology of the kidneys of alloxan-induced diabetes mellitus wistar rats model. Methodology: Thirty (30) adult male wistar rats were grouped into 6 (A-F) (n=5). Group A (control) received normal saline only. Group B (Untreated) received a single intraperitoneal injection of 150mg/kg of Alloxan. Group C, D, E and F received intraperitoneal injections of 150mg/kg of Alloxan, and then treated with 200, 300, 400 mg/kg of the MLEVA and 100mg/kg of Vitamin E respectively daily via oral route. The experiment lasted 16 days. The animals were sacrificed via a median abdominal incision, under ketamine (100mg/ml) as anesthesia 24 hours after their last treatment. The kidneys were carefully harvested, fixed and processed for routine H&E staining. Results: Treatment with MLEVA depicted a clear dose-dependent restoration of the kidney histoarchitecture evident by its tissue stroma appearing normal relative to the control, untreated and Vit E treated animal groups. Conclusion: MLEVA demonstrated a clear restorative potential on the kidney histoarchitecture after injurious changes resulting from alloxan-induced diabetes mellitus in wistar rat model. Keywords: Vernonia amygdalina, Diabetes Mellitus, Alloxan Nephropathy. Title: VERNONIA AMYGDALINA (BITTER LEAF) SHOWCASES RESTORATIVE POTENTIALS ON THE RENAL HISTOARCHITECTURE OF WISTAR RAT MODELS FOLLOWING ALLOXAN-INDUCED DIABETES MELLITUS Author: Christian Chiemeka Ozor, Afomachukwu Olivia Arimah, Chiadikobi Lawrence Ozoemena International Journal of Life Sciences Research ISSN 2348-313X (Print), ISSN 2348-3148 (online) Vol. 13, Issue 4, October 2025 - December 2025 Page No: 59-63 Research Publish Journals Website: www.researchpublish.com Published Date: 31-December-2025 DOI: https://doi.org/10.5281/zenodo.18109273 Paper Download Link (Source) https://www.researchpublish.com/papers/vernonia-amygdalina-bitter-leaf-showcases-restorative-potentials-on-the-renal-histoarchitecture-of-wistar-rat-models-following-alloxan-induced-diabetes-mellitus