Engineered PVA Hydrogel as a Universal Platform for Developing Stable and Sensitive Microbial BOD-Biosensors

Polyvinyl alcohol (PVA) hydrogels modified through radical polymerization under UV irradiation and Ce⁴⁺ ion treatment were investigated as a potential platform for developing highly sensitive biosensors for rapid biochemical oxygen demand analysis in water. These modifications enhance PVA physicochemical properties, including mechanical strength, stability, and biocompatibility, making it promising for immobilizing microorganisms in bioanalytical systems. A dual-mediator biosensor system using ferrocene (FC) and neutral red (NR) was developed with yeast <i>Blastobotrys adeninivorans</i> immobilized in modified PVA. The FC+NR-<i>B. adeninivorans</i>-PVA-Ce⁴⁺ system exhibited high sensitivity (linear range of 0.1-3.81 mgO₂/dm³), selectivity, and operational stability (up to 37 days service life), outperforming existing analogs. Testing with wastewater confirmed strong correlation with standard BOD₅, highlighting the potential for monitoring water quality. The described radical modification method is a simple and effective approach for creating sensitive and stable biosensors. It opens up new possibilities for environmental monitoring technology.