VANTAGE: van-based real-time HIV sequencing for transmission mapping and drug resistance profiling in war-affected Ukraine
We deployed the VANTAGE (VAN for Transmissible Agent Genomic Epidemiology) mobile system in Lviv, Ukraine, demonstrating end-to-end sequencing of dried blood spot samples within a clinic van usually serving de-occupied and frontline regions. HIV-1 pol sequences were obtained from 50% of samples, all subtype A6. Median time to 100× coverage was 38 min. Phylogenetic analysis revealed a local transmission cluster including a displaced person and the non-nucleoside reverse transcriptase inhibitor (NNRTI) resistance mutation E138A, supporting real-time HIV genomic surveillance in humanitarian crises.
Authors
- Mariia Liulchuk (ORCID: https://orcid.org/0000-0001-7689-4002)
- Pavlo Smyrnov (ORCID: https://orcid.org/0000-0003-4174-9432)
- Ganna Kovalenko (ORCID: https://orcid.org/0000-0002-4929-0841)
- Tetyana I. Vasylyeva (ORCID: https://orcid.org/0000-0002-9736-7022)
- Myroslava Filippovych (ORCID: https://orcid.org/0000-0002-6377-5271)
- Steffanie A. Strathdee (ORCID: https://orcid.org/0000-0002-7724-691X)
Institutions
- University of California, Irvine (US)
- University of California San Diego (US)
- L.V. Gromashevsky Institute of Epidemiology and Infectious Diseases of the National Academy of Medical Sciences of Ukraine (UA)
- Alliance for Public Health (UA)
Publication Details
- Journal
- AIDS
- Published
- 2025-12-04
- DOI
- https://doi.org/10.1097/qad.0000000000004360
- Primary Topic
- Forensic and Genetic Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00