Integrated Transcriptomic and Metabolomic Analysis Reveals the Meat Production Features in Hybrid Sheep

This study was conducted to investigate potential regulatory mechanisms of hybridization increased the meat production performance in sheep. Thirty-six 3-month-old male lambs of Suffolk sheep (SFK, <i>n</i> = 12), Hu sheep (HH, <i>n</i> = 12), and their F₁ hybrids (SH, <i>n</i> = 12) were selected and raised in individual pens under identical nutritional supply and husbandry management regimes over a 95-day (including a 15-day pre-trial period) experimental period. At the end of the feeding trial, six sheep closest to the average body weight were selected from each group for the subsequent trial, involving the collection of Longissimus dorsi samples and the determination of production performance, muscle fiber characteristic and transcriptomic and metabolomic analysis. The results showed that the SH sheep had significantly higher pre-slaughter live weight and carcass weight than the HH sheep, while lower than those of the SFK sheep (<i>p</i> < 0.05). The muscle fiber density of the SH group was significantly higher than that of the parental groups, while the muscle fiber diameter and cross-sectional area were significantly smaller (<i>p</i> < 0.05). The collagen fiber content of the SH group was intermediate between the two parental groups and significantly higher than that of the SFK group (<i>p</i> < 0.05). Transcriptomic analysis identified 2920 differentially expressed genes (DEGs), which were mainly enriched in the AMPK, PI3K-Akt, and PPAR signaling pathways. Metabolomic analysis detected 1617 differential metabolites (DMs), which were enriched in the fatty acid degradation and steroid hormone biosynthesis pathways. Integrated analysis revealed that core genes <i>SESN3</i> and metabolites (malate, testosterone) enhance energy supply capacity through AMPK pathway, thereby promoting muscle fiber proliferation and increasing meat yield in the hybrid sheep. In conclusion, the heterosis of the SH group originates from the remodeling of muscle fiber structure and the synergistic regulation of related pathways, which provides a theoretical basis for sheep crossbreeding.