Isolation and genomic analysis of secondary metabolism in cave Actinomycetota from biofilms and Ceuthophilus

We report the isolation and genomic sequencing of nine Actinomycetota obtained from both cave biofilms and trogloxenic <i>Ceuthophilus</i> (cave crickets). Strains were isolated from samples via actinomycetota-selective media and sequenced using nanopore sequencing. Provisional taxonomic inference of strains was performed by whole genome phylogenetic analysis, revealing a broad range of genera, with most strains clustering with known genera with digital DNA-DNA hybridization values less than 70%, and one strain not clustering with known genera. An analysis of secondary metabolism reveals the quantity and diversity of secondary metabolism in cave Actinomycetota.