The 2025 Report on the Human Proteome from the HUPO Human Proteome Project
The HUPO Human Proteome Project (HPP) aims to complete the human protein parts list by detecting evidence of expression and of function for all proteins in the human proteome, and make proteomics an integral part of multiomics studies of health and disease. Here we describe the state of the 2025 HPP reference proteome of 19,435 proteins, based on GENCODE v48, UniProtKB 2025_03, Human Protein Atlas 24, MassIVE-KB 2023, and PeptideAtlas 2025-01. We evaluate the progress in the past year, with 93.6% of the proteome detected, and examine the proteins that have not yet been detected to determine where further progress can be made. We also evaluate the progress in determining at least one function for every protein in the HPP target list, finding an increase of 288 proteins in the highest category (FE1) to 5562. Finally, we provide highlights from 12 Biology/Disease-based HPP initiatives, HPP resource pillars, and π-HuB.
Authors
- Susan T. Weintraub (ORCID: https://orcid.org/0000-0002-8328-7814)
- Jennifer E. Van Eyk (ORCID: https://orcid.org/0000-0001-9050-148X)
- Gilbert S. Omenn (ORCID: https://orcid.org/0000-0002-8976-6074)
- Bogdan Budnik (ORCID: https://orcid.org/0000-0003-3622-2003)
- Sandra Orchard (ORCID: https://orcid.org/0000-0002-8878-3972)
- Cecilia Lindskog
- Edouard C. Nice (ORCID: https://orcid.org/0000-0001-5480-4715)
- Claudia Ctortecka (ORCID: https://orcid.org/0000-0002-6886-8936)
- Robert L. Moritz (ORCID: https://orcid.org/0000-0002-3216-9447)
- G. Zhang
- Nicolle H. Packer (ORCID: https://orcid.org/0000-0002-7532-4021)
- Eric W. Deutsch (ORCID: https://orcid.org/0000-0001-8732-0928)
- Ruedi Aebersold
- Zhi Sun (ORCID: https://orcid.org/0000-0003-3324-6851)
- Uwe Völker (ORCID: https://orcid.org/0000-0002-5689-3448)
- Nuno Bandeira (ORCID: https://orcid.org/0000-0001-8385-3655)
- Overall Christopher M.
- Chenfei Zheng (ORCID: https://orcid.org/0009-0005-7154-7526)
- Charles Pineau
- Marie Andken
- Michael H. A. Roehrl
- Jonathan M. Mudge
- Tiannan Guo
Institutions
- Uppsala University (SE)
- European Bioinformatics Institute (GB)
- Harvard University (US)
- University of British Columbia (CA)
- Inserm (FR)
- École des Hautes Études en Santé Publique (FR)
- The University of Texas at San Antonio Health Science Center (US)
- University of Michigan (US)
- Universitätsmedizin Greifswald (DE)
- Hadassah Medical Center (IL)
- Australian Regenerative Medicine Institute (AU)
- Harvard University Press (US)
- Westlake University (CN)
- Institute for Systems Biology (US)
- ETH Zurich (CH)
- University of California San Diego (US)
- Abterra Biosciences (United States) (US)
- Key Laboratory of Guangdong Province (CN)
- InSysBio (Russia) (RU)
- IPM Institute of North America (US)
- Institut de Recherche en Santé, Environnement et Travail (FR)
- German Centre for Cardiovascular Research (DE)
- Department of Biological Sciences (RU)
- Bioinformatics Institute (RU)
- Cedars-Sinai Smidt Heart Institute (US)
- Cancer Genetics (United States) (US)
- Inspire Institute (US)
- Université de Rennes (FR)
- Monash University (AU)
- Macquarie University (AU)
Publication Details
- Journal
- Journal of Proteome Research
- Published
- 2026-01-05
- DOI
- https://doi.org/10.1021/acs.jproteome.5c00759
- Primary Topic
- Advanced Proteomics Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00