Quantitative Phytochemical Analysis and Bioactivities of Hydroethanolic Extracts of <i>Amorphophallus consimilis</i>

This study is devoted to the determination of polyphenol, flavonoid and mineral contents and then to the evaluation of antioxidant and antibacterial activities of Amorphophallus consimilis extracts. The spectrophotometric method is used for the determination of polyphenol and flavonoid contents with standard solutions of gallic acid and quercetin respectively. The polyphenol content varies from 2.33 (stems) to 4.68 (tubers) µg EAG/g and that of flavonoids from 2.425 (tubers) to 9.373 (stems) µg EQ/g. The antioxidant activity is evaluated by the DPPH<sup>•</sup> and ABTS<sup>+</sup> method. The 50% Inhibitory Concentration (IC<sub>50</sub>) values obtained with both methods range from 0.17±0.011 (leaves) to 0.254±0.001 (tubers) mg/mL. Atomic absorption spectrophotometry (AAS) is used for the determination of mineral contents. <i>Amorphophallus consimilis</i> contains minerals with average contents of calcium (43 µg/g), iron (11 µg/g), zinc (0.28 µg/g) and copper (0.14 µg/g). The disk diffusion method is used for the evaluation of antibacterial activity. A total of five bacterial strains are used, these are: <i>Escherichia coli</i> <i>ATCC25922</i>, <i>Enterococcus faecalis</i> <i>ATCC29213</i>, <i>Staphylococcus aureus</i> <i>ATCC29212</i>, <i>Pseudomonas</i> (community strain), <i>Candida albican ATCC24433</i> were used. The minimum inhibitory concentrations (MIC) range from 1.875 to 30 mg/mL, showing overall interesting bacterial activity of the extracts on the strains tested.