Profiling DNA-protein interactions in Meloidogyne incognita using dCas9-based affinity purification
The optimized in vitro dCas9-based CAPTURE protocol offers a new tool for investigating chromatin dynamics and regulatory proteins in non-transformable nematodes. This method expands the scope of effector gene regulation research and provides new insights into M. incognita parasitism. Future research will aim to validate these regulatory proteins and extend the method to other effector loci, potentially guiding the development of novel nematode control strategies.
Authors
- Caroline Bournaud (ORCID: https://orcid.org/0000-0002-2980-1691)
- Yohann Couté (ORCID: https://orcid.org/0000-0003-3896-6196)
- Sebastian Eves-van den Akker
- Alwéna Tollec
- Etienne G. J. Danchin
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Inserm (FR)
- Université Côte d'Azur (FR)
- University of Cambridge (GB)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- CEA Grenoble (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Laboratoire de Microbiologie, Adaptation et Pathogénie (FR)
- Institut Sophia Agrobiotech (FR)
- Université Grenoble Alpes (FR)
Publication Details
- Journal
- Plant Methods
- Published
- 2026-01-04
- DOI
- https://doi.org/10.1186/s13007-025-01475-5
- Primary Topic
- Nematode management and characterization studies
- Type
- article
- Field-Weighted Citation Impact
- 0.00