Comparative oral and dermal bio accessibility study of Ayurvedic Antidote (Manjishthadi Agada) by Franz Diffusion method

Background & objectives: Manjishtadi Agada described in Bharat Bhaishajya Ratnakar is indicated in all type of poisoning but the route of administration is not mentioned. Agada are used mainly from most of the routes of administration like internally (excluding parental routes) and externally in poisoning condition. In emergency condition like poisoning or snake bite, fast absorption of the drug is needed to achieve its peak and effect. The present study was conducted to compare the absorption of Manjishtadi Agada through oral and dermal route. Methods: In this study, Manjishtadi Agada was prepared which was subjected to physicochemical analysis and dermal as well as oral bio accessibility study with the help of Franz cell diffusion apparatus. The absorbance and its time were recorded and the graphs were plotted for dermal and oral bio accessibility of Manjishthadi Agada. Assessment was done with the help of calibration curve to estimate the absorption of the drugs with different time interval and dilutions. Pearson’s Correlation coefficient was estimated to observe the correlation between rate and time of absorption of both the routes. Results: It was observed that there is statistically significant difference in the absorbance of Manjishthadi Agada through dermal and oral route. Interpretation and Conclusions: Bio accessibility of Manjishtadi Agada is more with oral route than that of dermal route. Manjishthadi Agada may be absorbed earlier when administered through oral route at higher concentration. In acute and chronic condition of poisoning, it should be administered through oral route.