Multi-drug resistant and extended-spectrum β-lactamase producing Klebsiella pneumoniae from clinical samples

This study aimed to determine the MDR and ESBL producing K. pneumoniae from clinical samples. Laboratory based cross-sectional study was conducted from the samples obtained from the patients of Tribhuvan University Teaching Hospital. Antibiotic susceptibility test was performed and ESBL was detected through the combination disk method. Of the 377 K. pneumoniae isolates, 304 (80.6%) were MDR and 279 (74%) exhibited ESBL activity. Significant percentages of MDR were detected in blood (p=0.004) and urine (p=0.058) whereas ESBL in urine (p=0.026). Most ESBL-producing K. pneumoniae exhibited resistance to ß-lactams, quinolones, aminoglycosides and macrolids. This suggests for strict adherence to antimicrobial susceptibility results for the treatment and implementation of antibiotic stewardship programme.