Reconstituting the head and neck tumor microenvironment with air-liquid interface organoids

An <i>in vitro</i> HNC immune microenvironment model was successfully constructed using the ALI method. This model maintained the proportions and structures of the components of the original tumor, such as tumor-infiltrating lymphocytes and cancer-associated fibroblasts, for a period of time <i>in vitro</i>, providing an experimental platform for exploring the complex crosstalk between HNC cells and multiple cell colonies. This study preliminarily validated the feasibility of using ALI organoid models to evaluate the efficacy of immunotherapy drugs in treating HNC, providing a reliable and stable preclinical model, and new ideas for drug screening platforms for personalized precision medicine in HNC.