Immunoregulatory Bioinspired Hydrogel from Decellularized Amnion for Infected Wound Healing

Infectious wounds remain a formidable clinical challenge due to persistent bacterial colonization, uncontrolled inflammation, and impaired tissue regeneration. To address these issues, we developed a multifunctional immunoregulatory hydrogel by integrating curcumin (Cur) loaded polydopamine (PDA)-coated zeolitic imidazolate framework-8 (ZIF-8) nanoparticles (Cur/ZIF/PDA) into a photo-cross-linkable hydrogel matrix derived from decellularized human amniotic membrane (dECMMA). This bioinspired hydrogel (dECMMA/ZIF/PDA) exhibited enhanced photothermal antibacterial activity, controlled drug release, and excellent biocompatibility. <i>In vitro</i> and <i>in vivo</i> studies demonstrated that the hydrogel not only efficiently eradicated bacteria but also promoted macrophage polarization toward an anti-inflammatory M2 phenotype, upregulated angiogenesis-related gene expression, and accelerated wound re-epithelialization and collagen remodeling. Notably, the synergistic action of photothermal therapy and immunomodulation significantly improved healing outcomes in a <i>Staphylococcus aureus</i> (<i>S. aureus</i>) infected wound model. This study presents a promising strategy for chronic wound management by leveraging naturally derived extracellular matrix components and multifunctional nanocarriers to orchestrate immune microenvironment remodeling and tissue regeneration.