Investigating the transcriptional fingerprints of cocultured Saccharomyces cerevisiae and Lachancea thermotolerans in a model wine environment

The results highlight pathways and genes consistently enriched in all studies, including copper ion import, transition metal and iron ion transport, cell wall mannoproteins and biogenesis as well as methionine and sulfur biosynthesis. Interestingly, <i>FIT2</i> deletion in the original wine yeast wildtype strain (VIN13Δ<i>fit2</i>) showed opposite, but still interaction specific, phenotypes when compared with the laboratory strains of the Euroscarf deletion library. Considering the evolutionary context of these strains and likely differences in cell wall mannoprotein composition, these data emphasize the challenges of gene annotation in an ecosystem relevant context. The findings reinforce observations from previous research, suggesting that <i>FIT2</i> has a significant role in modulating interactions between species and highlighting specific DEGs from pathways that require further investigation in future coculture studies.