Вплив таблеток з півонії коренів екстрактом сухим, L-триптофаном і гліцином на стан головного мозку за умови експериментальної черепно-мозкової травми

The aim of this work was to experimentally substantiate the neuroprotective potential of new combined tablets containing dry extract of peony roots, L-tryptophan, and glycine to eliminate the existing gap in the pharmacotherapy of traumatic brain injury (TBI) associated with the insufficient effectiveness of current monotherapeutic approaches. Material and methods. The study was conducted on 40 male white rats weighing 200–250 g. The TBI model was induced by the free fall of a weight onto a fixed head of the animal. The test preparation was administered orally for 7 days. The effectiveness was assessed using morphological, morphometric, and biochemical methods, including the determination of neuron-specific enolase (NSE) and S100 protein levels in blood serum, as well as analysis of the condition of neurons in the sensorimotor cortex and hippocampus. Results. Rats with TBI demonstrated a significant increase in NSE and S100 levels, a decrease in the number of normochromic neurons, activation of glial cells, and cerebral tissue oedema. Administration of the combined drug contributed to a reduction in neuronal damage markers, a decrease in the glio-neuronal index, and normalization of brain microstructures. Morphological examination revealed preservation of the neuronal layer and a reduction in destructive changes, indicating a pronounced neuroprotective effect of the drug. Conclusions. The developed combined drug demonstrated an effective protective effect on brain tissue under conditions of experimental TBI. The obtained results substantiate the feasibility of further preclinical studies to investigate its mechanisms of action and potential clinical application