Periodontopathogenic and Cariogenic Microbiota in Patients With and Without Oral Cancer

Objectives: To determine the frequency and abundance of periodontopathogenic and cariogenic bacteria in patients with oral cancer and controls. Methods: Descriptive cross-sectional study in plaque and saliva of 10 patients with oral cancer and 10 controls. DNA was sequenced using the Illumina MiSeq™ 2500 and analyzed with bioinformatics tools. 140 periodontal pathogenic and 111 cariogenic bacteria were studied. Frequency analysis, inter- and intra-group comparisons, and Chi-square were performed. Results: Periodontopathogenic bacteria Treponema denticola and Campylobacter concisus were frequent and abundant in patient plaques; Prevotella melaninogenica, Veillonella parvula, Streptococcus mutans, Streptococcus cristatus, Streptococcus mitis, Streptococcus constellatus, Prevotella intermedia, Aggregatibacter actinomycetemcomitans were frequent and abundant in control saliva. Veillonella parvula, T. denticola, Streptococcus sanguinis, Streptococcus salivarius, Streptococcus parasanguinis, S. mutans, S. mitis, Streptococcus gordonii, Streptococcus cristatus, S. constellatus, Streptococcus anginosus, Rothia mucilaginosa, Pseudomonas aeruginosa, Prevotella melaninogenica, intermedia Porphyromonas gingivalis, Olsenellauli, Lactobacillus gasseri, C. concisus, Aggregatibacter aphrophilus, A. actinomycetemcomitans, and Achromobacter xylosoxidans were frequent in plates from patients than from controls. P. melaninogenica, P. gingivalis, Leptotrichia buccalis, and Haemophilus parainfluenzae were common in saliva from patients, and S. mutans and R. mucilaginosa were common in saliva from controls. In patients, cariogenic bacteria such as H. parainfluenzae, S. sanguinis, Tannerella forsythia and V. parvula were abundant in plaque. Capnocytophaga ochracea, H. parainfluenzae, P. denticola, T. forsythia were abundant in saliva. In controls L. buccalis, Prevotella denticola, Selenomonas sputigena, S. cristatus, Streptococcus oralis were abundant in plaque and P. melaninogenica, R. dentocariosa, R. mucilaginosa, S. mitis, S. oralis, S. salivarius, S. sanguinis and V. parvula were abundant in saliva. Conclusions: Treponema denticola, Campylobacter concisus were frequent and abundant in plaque from patients compared to controls. Streptococcus mutans, R. mucilaginosa were frequent and abundant in saliva of controls compared to patients.