Physiological effects of cobalt nanoparticles by using the alcoholic extract of Artemisia herba alba on laboratory rats induce diabetes

In the present study, 30 male laboratory rats were divided into five groups to examine the effects of cobalt nanoparticles(CoNPs) coated with Artemisia herba alba, Artemisia herba alba nanoparticles, and alcoholic extract of Artemisia herba alba on diabetic rats on body weight, organs' weight, and selective hematological parameters compared with the untreated group. The control group was injected (I.P) with normal saline (0.9%), and the second group was injected with alloxan (150 mg/kg). For 30 days of treatment, the third group was treated by CoNPs coated with Artemisia herba alba ( 104.5µg\ml) , whereas the fourth and fifth groups were treated with Artemisia herba alba nanoparticles (146µg\ml) and Artemisia herba alba extract (500 mg/kg), respectively. The results show that 30 days of treatment with CoNPs coated with A. herba alba, Artemisia herba alba nanoparticles and the extract of Artemisia herba alba, there is a significant increase (P≤0.05) in RBC (6.61±0.24, 6.75±0.19, 7.04±0.38) respectively, compared with the untreated group (4.22±0.48), also there is a significant increase (P≤0.05) in HB and PCV (10.66 ± 0.38, 32.03 ± 1.06) whereas there is a significant decrease (P≤0.05) in WBC (8.75 ± 0.75) when treated with CoNPs coated with Artemisia herba alba (8.75±0.75 ). The results of the current study indicate that treating with cobalt nanoparticles coated with Artemisia herba alba extract has a significantly greater therapeutic effect on diabetic rats compared with other interventions tested.