Wound Healing Potential of Hydrogel Extract of <i>Saccharum spontaneum</i> Leaves Using Incision Wound Healing Model in Rats

Saccharum spontaneum is a medicinal plant known for treating various diseases, such as wounds. Despite the potential use of this plant, there are no studies confirming its wound-healing activity. This study assessed S. spontaneum extract hydrogel formulation (HESs) and examined its effects on wound healing, antioxidant properties, and antibacterial activity. The disk diffusion method was used to evaluate the antibacterial activity, while DPPH and FRAP were used to measure the antioxidant activity. The rats were divided into 5 groups of 4 rats each to assess wound healing using the incision wound model. Group 1 served as the negative control, while group 2 was given 0.05% octenidine. S. spontaneum extract hydrogel formulation (HESs) was administered to groups 3, 4, and 5 at 5%, 10%, and 15% (%w/w) concentrations, respectively. The results showed that S. spontaneum extract has antioxidant activity with IC50 values of 80.16 (in DPPH) and 82.70 µg/mL (in FRAP), while exhibiting an inhibitory effect on the growth of bacteria in a concentration-dependent manner. The wound healing activity test results indicated that 10% and 15% HESs significantly closed the wound area on days 4, 8, and 16. The S. spontaneum leaf extract exhibited wound healing potential.