Mutations of six amino acid residues in a B domain-deleted blood coagulation factor VIII have a cumulative effect on increasing its secretion
Combining the 6 mutations in BDD-FVIII improved its expression and did not affect general protein properties, making it promising for future product development. The data also indicate that BDD-FVIII and FL-FVIII have different expression mechanisms.
Authors
- Elizabeth Del Greco
- Vijaya L. Simhadri (ORCID: https://orcid.org/0000-0002-3177-5250)
- Margarita Krivega (ORCID: https://orcid.org/0000-0003-0478-7122)
- Pratima Bajgain
- Andrey G. Sarafanov (ORCID: https://orcid.org/0000-0001-6124-9713)
- Haarin Chun (ORCID: https://orcid.org/0000-0002-2668-6296)
- Svetlana A. Shestopal (ORCID: https://orcid.org/0000-0003-0725-5885)
- Leonid A. Parunov (ORCID: https://orcid.org/0000-0002-8892-8908)
- Yideng Liang (ORCID: https://orcid.org/0000-0002-2915-3364)
- Zuben E. Sauna (ORCID: https://orcid.org/0000-0001-8202-5188)
- Wojciech Jankowski (ORCID: https://orcid.org/0000-0002-7000-9057)
- Philip Olivares
- Julia Poniatowski
- Ivan Krivega
- Wells W. Wu
- Bo Shi
- Abu Hasanat Md Zulfiker
Institutions
- Center for Biologics Evaluation and Research (US)
- Calithera (United States) (US)
Publication Details
- Journal
- Research and Practice in Thrombosis and Haemostasis
- Published
- 2025-12-31
- DOI
- https://doi.org/10.1016/j.rpth.2025.103325
- Primary Topic
- Hemophilia Treatment and Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00