Application of the auxin-inducible degron 2 (AID2) system to analyze stage-specific roles of the histone variant H2A.Z during development of <i>Caenorhabditis elegans</i>
Multiple roles of the evolutionarily conserved histone variant H2A.Z in development have been proposed. However, conventional H2A.Z knockouts cause embryonic lethality. Here, we developed a transient depletion system for H2A.Z in Caenorhabditis elegans using an auxin-inducible degron and demonstrated its contribution to germline differentiation at early developmental stages. This system can be applied to investigate temporal protein functions during development.
Authors
- Natsumi Horii
- Masato T. Kanemaki (ORCID: https://orcid.org/0000-0002-7657-1649)
- Asako SUGIMOTO
- Saho Kitagawa
- Masahiko Harata
- Yukako Oma
- Haruta Nami
Institutions
- National Institute of Genetics (JP)
- Tokyo University of Science (JP)
- The Graduate University for Advanced Studies, SOKENDAI (JP)
- Tohoku University (JP)
- The University of Tokyo (JP)
Publication Details
- Journal
- Bioscience Biotechnology and Biochemistry
- Published
- 2025-12-31
- DOI
- https://doi.org/10.1093/bbb/zbaf200
- Primary Topic
- Genomics and Chromatin Dynamics
- Type
- article
- Field-Weighted Citation Impact
- 0.00