Green synthesis of gold nanoparticles using extracted, purified flavonoid from Myrtus communis Leaves.

Background: The parts of Myrtus communis ( known as Al-Yas) were collected in Oct. 2023 from several areas in Baghdad, Iraq. Methodology: An aqueous extract was prepared using the traditional method, and gold nanoparticles were subsequently synthesized from the plant extract. Four different concentrations of AuNPs were prepared. Various methods are used to characterize green-manufactured gold nanoparticles and to determine phenol content. The Folin Ciocalteu assay method and the Aluminum chloride method were used for flavonoid determination. Results: The total phenolic content, expressed as gallic acid equivalents, was determined using the following equation, where the phenolic concentration was 33.4 mg/g. According to the straight-line equation, the total flavonoids were 17.66 mg / g of the extracted total flavonoids. A change in the extract color due to a reduction reaction serves as the first indicator for the green synthesis of gold nanoparticles. Additionally, results showed that the diameter of the extract molecules was 253.0 nm, while it was 65.27 nm for green-synthesized AuNPs from crude extract and 44.99 nm for AuNPs from flavonoid. UV-Visible spectroscopy of aqueous extract was absorbed at 285 nm, and it was 550 nm for green synthesized AuNPs from crude extract, while it was 555 nm for AuNPs for flavonoid. Also, FE-SEM images revealed differences in the characteristics of crude extracts and AuNPs derived from crude extracts or from purified flavonoids. Conclusion: These results were promising for obtaining nanoparticles with unique properties via a green method that is environmentally safe.