Enhancing 1,5-Pentanediamine Productivity in Corynebacterium glutamicum with Improved Lysine and Glucose Metabolism

1,5-Pentanediamine (PDA) is an important monomer for the synthesis of nylon materials. However, its microbial production from glucose is severely limited by product cytotoxicity, which slows the metabolism of both precursor lysine and glucose uptake. To overcome this limitation, a PDA-responsive dynamic regulatory switch (PDRS) was constructed using the transcriptional repressor CgmR and the PcgmA promoter. By replacing promoters and ribosome-binding sites, the response window of the PDRS was optimized to a PDA concentration range of 38.9–87 g/L. Based on this system, the PDRS was employed to enhance lysine biosynthesis and glucose uptake. Following fermentation optimization, the optimal strain Corynebacterium glutamicum YY3.6 produced 105.5 g/L PDA within 36 h, achieving a PDA productivity of 2.93 g/L/h and a yield of 0.36 g/g glucose. Collectively, these results provide an effective strategy for the microbial production of PDA from glucose.