Cellular-level effects of a newly developed low-intensity pulsed ultrasound device on wound healing: An experimental study using the L929 fibroblast cell line

This study aimed to investigate the proliferative effects of a newly developed low-intensity pulsed ultrasound (LIPUS) device, produced in Türkiye, on the healthy L929 mouse fibroblast cell line. L929 fibroblast cells were exposed to LIPUS at a frequency of 1.5 MHz and a power density of 0.5 W/cm 2 . The application was performed for 20 minutes daily over periods of 24, 48, and 72 hours. At each time point, cell viability was assessed using the MTT assay, and morphological structures were evaluated using an inverted microscope. Each condition was tested in triplicate. LIPUS application significantly increased fibroblast proliferation, particularly at 24 and 48 hours of incubation (p<0.01, p<0.001). Although an increase was also observed at 72 hours, it was not statistically significant. Microscopic analysis revealed no degenerative or cytotoxic changes in cell morphology. The cells maintained their natural adhesion and division characteristics. The newly developed LIPUS device significantly enhanced fibroblast proliferation at 24 and 48 hours without affecting cell morphology, supporting its potential as a safe and effective biophysical tool for tissue healing.