Effect of hydrogel with a new acexamic acid derivative on the survival of ischemic tissue

Introduction: Prevention and correction of the consequences of local ischemia developing during various surgical interventions is a pressing issue in modern medicine. Materials and Methods: The study was conducted on 24 Agouti viable yellow mice weighing 30-35 g. An ischemic skin flap measuring 10 x 30 mm was created on the back skin of mice with a mutation in the Agouti viable yellow gene. Immediately after the procedure, the animals were topically treated twice daily with a hydrogel containing 2-ethyl-6-methyl-3-hydroxypyridinium N-acetyl-6-aminohexanoate (2E-6M-3G-NA-6A) (5%) and reference drug, panthenol gel (10%) for 10 days. Wound healing was monitored using planimetry, microcirculation, and histomorphological and biochemical parameters. Results: The efficacy of a hydrogel based on 2E-6M-3G-NA-6A on skin tissue reparation processes was studied in transgenic C57BL/6J mice with a dominant mutation in the Agouti yellow (Au/a) gene using an isolated pedicle skin flap model. Topical application of 5% hydrogel based on (2E-6M-3G-NA-6A) twice daily for 10 days resulted in a 1.6-fold decrease in the area of tissue necrosis compared to that in the control group without pharmacological support. On the 10th day of the experiment, in the group of animals treated with the hydrogel based on 2E-6M-3G-NA-6A, an increase in the level of microcirculation was observed on average to 489.1 ± 29.1 perfusion units (PU) (p = 0.0001) compared to such in the group of animals without treatment – 242.3 ± 19.6 PU (p < 0.0018). Hydrogel based on 2E-6M-3G-NA-6A, when applied topically to an ischemic wound, led to an increase in the level of alkaline phosphatase on the 7th day to values of 157.4 ± 12.7 (p < 0.0014). U/L, which is 1.8 times higher than in the control group. Conclusion: 2E-6M-3G-NA-6A in a hydrogel dosage form is capable of stimulating wound healing in ischemic tissues to the level of non-ischemic wounds and may become a new and effective method for treating long-term non-healing wounds.