Missing steps of mitochondrial translation initiation identified in plants
Translation initiation, the first step of the translation cycle, involves initiation factors (mtIF) 2 and 3 in mitochondria. mtIF3 release from the ribosomal small subunit was believed to be a prerequisite for the recruitment of the initiator tRNA. Here we use cryogenic electron microscopy to characterize plant mitochondria pre-initiation complexes and to reveal how plant mtIF3 binds the initiator tRNA and facilitates its accommodation in the decoding center.
Authors
- Philippe Giegé (ORCID: https://orcid.org/0000-0003-2285-8190)
- Heddy Soufari (ORCID: https://orcid.org/0000-0003-3434-2917)
- Vasileios Skaltsogiannis (ORCID: https://orcid.org/0000-0003-4155-9540)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Université Paris-Saclay (FR)
- Synchrotron soleil (FR)
- Institut de Biologie Intégrative de la Cellule (FR)
- Institut de Biologie Moléculaire des Plantes (FR)
- CEA Paris-Saclay (FR)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2025-12-30
- DOI
- https://doi.org/10.64898/2025.12.30.697032
- Primary Topic
- Mitochondrial Function and Pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Universität Basel
- Equipex
- Agence Nationale de la Recherche
- Université de Strasbourg
- Centre National de la Recherche Scientifique
- French Infrastructure for Integrated Structural Biology