Histidine methylation <i>via</i> an enzymatic cascade with <i>in situ</i> generation of nucleoside-modified AdoMet analogues
Histidine methyltransferases (HMTs) are AdoMet-dependent enzymes that catalyse regioselective methylation of histidine residues in proteins. Here, we demonstrate that a methionine adenosyltransferase (MAT)/HMT cascade system enables the generation and utilisation of AdoMet analogues for efficient and selective histidine methylation in peptide substrates.
Authors
- Jasmin Mecinović (ORCID: https://orcid.org/0000-0002-5559-3822)
- Andrea Rentmeister (ORCID: https://orcid.org/0000-0002-3107-4147)
- Jakub Drożak (ORCID: https://orcid.org/0000-0002-3601-3845)
- Marija Ram
- Arne Hoffmann (ORCID: https://orcid.org/0009-0004-8635-0657)
- Antonia L. Irion
- Zhimei Yu
Institutions
- University of Southern Denmark (DK)
- University of Warsaw (PL)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-12-30
- DOI
- https://doi.org/10.1039/d5cc06375b
- Primary Topic
- Cancer-related gene regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.00