ACTIVITIES OF CMC-ASE AND β-GLUCOSIDASE IN CELLULOLYTIC BACTERIA FROM BUFFALO RUMEN GROWN ON CARBOXYMETHYL CELLULOSE

Cellulase is an enzyme widely used in the feed and food sectors.This study aims to examine the CMC-ase and -glucosidase activity of cellulolytic bacteria in degrading cellulose content in CMC substrate and determine the optimum incubation period to produce cellulase enzymes from buffalo rumen bacteria.This study used a factorial Completely Randomized Design (CRD) consisting of 2 factors and 4 replications.The first factor is the type of bacterial isolate consisting of 2 types, namely ST6 and ST8.The second factor is the difference in the incubation period consisting of 5 treatments (T0: 0 days, T1: 3 days, T2: 6 days, T3: 9 days, and T4: 12 days) on the use of 1% CMC (Carboxy Methyl Cellulose) substrate.The data were analyzed using variance (ANOVA) and further tested using Duncan's Multiple Range Test (DMRT).The results of data analysis showed that CMC-ase activity was influenced by the incubation period with the best incubation period at 9 days (T3), and there was a significant interaction between the type of isolate and the incubation period.The activity of the -glucosidase was influenced by the type of isolate and the incubation period, with the best results at an incubation period of 6 days.The interaction between the isolate type and the incubation period significantly affected the activity of the -glucosidase.