Development and analysis of PVC glass slides for urine microstructure studies

• A transparent PVC film stabilized with p-chlorobenzamidine exhibits significantly reduced photodegradation kinetics and suppressed chain scission under UV exposure. • The PVC+L system preserves molecular integrity, surface morphology, and optical clarity, enabling reliable urine sediment preservation for up to one year under ambient conditions. • Comprehensive kinetic, spectroscopic, and morphological analyses demonstrate that effective photostabilization can be achieved using a low-loading organic additive without metal-based stabilizers. The long-term maintenance of urine precipitation microstructures stays a main restriction in medical laboratory education and routine urinalysis, as traditional glass sides typically permit sample stability for only 1–3 h. In this work, a novel polyvinyl chloride (PVC)-coated glass slide system was advanced to capable long-term stabilization and microscopic conception of urine precipitation components. PVC films were functionalized with p-chlorobenzamidine (L) as photostabilizer to inhibit photodegradation induced via elongate exposure to UV light and microscope. The films photostability were systematically investigated employing FTIR, viscosity measurements, UV–Vis and FESEM analysis. At 365 nm, after 125 h of UV irradiation, the carbonyl index (Ico) for PVC+ L films were increased to 0.13, compared with 0.18 of PVC, suggesting an essential reduction in oxidative degradation. The constant rate of photodegradation (kd) was reduced from 0.0021 s -1 for pristine PVC to 0.0014 s -1 for PVC+ L sample, while the quantum yield of main-chain scission (Φcs) was decreased from 373×10 13 to 884×10 12 . Moreover, the PVC+ L films preserved a higher viscosity-average molecular weight (Mv 260,000) contracted with the pristine PVC film after protracted irradiation, suggesting efficient inhibition of PVC chain scission. Functionally, at 295 nm, the PVC+ L films appeared high (90 % transmittance) and cement clear microscopic conception of urine precipitation components. Cystine crystals, uric acid, calcium oxalate and triple phosphate, as well as amorphous materials, epithelial cells, kept optically distinguishable and morphologically intact for up to one year. These finds illustrate, for the first time, that photostabilized films can use as transparent, durable and chemically stable substrates for urine precipitation preservation, giving a low-cost and practical platform for routine microscopy, medical laboratory training and educational characteristics.