Microdosimetry of 3D Virtual Stem Cells Inside an Electro-Pulsed Biohybrid Device for Spinal Cord Regeneration

Within the RISEUP project, an implantable electro pulsed biohybrid (EPB) device is being developed to promote neurogenesis from mesenchymal stem cells (MSCs) and induced neuronal stem cells (iNSCs), through intense ultrashort pulsed electric field (<tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$\mu$</tex> sPEFs) stimulation for spinal cord injury (SCI) regeneration. Such a stimulation modulates intracellular calcium fluxes by enhancing the permeability of cellular compartments membranes, to boost stem cell proliferation and differentiation in neuronal lineage. In this framework, microdosimetric techniques, combined with advanced computational models, are essential for simulating in vitro experimental conditions, to deepen <tex xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">$\mu$</tex> sPEFs' biophysical effects on cells. In this work, the μsPEFs stimulation microdosimetry will be performed in Comsol Multiphysics (v. 6.1) recreating a mixture of 3D virtual stem cells, including subcellular structures and internal organelles, hosted inside the EPB.