Different plasma exosome isolation methods generated distinct microRNA and protein profiles in healthy controls and patients with advanced prostate and lung cancer

The choice of exosome isolation method profoundly influences molecular profiling, with SEC optimizing miRNA detection, lectin-binding enhancing protein capture, and TIM4-binding enriching cancer-specific miRNAs. These findings underscore the need for tailored isolation strategies to unlock exosomes' potential as precise, multi-omic biomarkers for cancer diagnosis and monitoring.