Prevalence Of Fluroquinolone Resistant Escherichia coli Isolates From Urine Samples

Background: Escherichia coli accounts for the vast majority of the gram-negative urinary tract infections clinically that are traditionally managed using flouroquinolones antimicrobial agents ; Ciprofloxacin is the commonest flouroquinolones used for treating urinary tract infection. Fluoroquinolone resistance in Escherichia coli is evolving as a crisis because of the non-properly used antibiotics, that resulted in antibiotic resistance. Plasmid-mediated resistance might be transferred horizontally among the Enterobacteriaceae family, that might cause more dissemination of antibiotic-resistance genes within various reservoir. Methods: Over a six-month duration, a total of 100 isolates including 49 E. coli isolates were collected. Antibiotics susceptibility was detected using Kirby–Bauer disk diffusion approach based on CLSI thereafter by MIC. Ultimately , resistant isolates to flouroquinolones were tested for determination of qnr A gene using PCR technique. Results: Among 49 E.coli isolates (73.5%) isolates were resistant to flouroquinolones;E.coli isolates that were resistant to ciprofloxacin was 69.4% by MIC with nearly the same percentage by disc diffusion 61.2%. The highest percentage of E.coli isolates were resistant to Ciprofloxacin6(61.2%) followed by Nalidixic acid and levofloxacin ( 55.1%), Norfloxacin (49%), Amikacin (34.7%), cefoxitin( 32.7%), Sulphmethoxazole-Trimethoprim (20.4%), Nitrofurantoin ( 8.2%) then Amoxclav acid( 6.1%). Qnr A gene prevalence in this study were lacking in 100% of E. coli samples.