Mechanistic insights into Rottlerin’s inhibition of MrkH-mediated biofilm and capsule formation in Klebsiella pneumoniae

These results substantiate the function of Rottlerin as a non-activating competitive binder, providing mechanistic insight into its potential as an anti-biofilm agent and building a foundation for the rational design of small-molecule inhibitors aimed at c-di-GMP regulatory pathways. Our findings demonstrate that Rottlerin is a potent and efficient sub-MIC inhibitor of K. pneumoniae's ability to form biofilms and capsules.