Validated HPTLC analysis method for quantification of variability in phytochemicals present in the leaf of <i>Moringa oleifera</i> Lam. Collected from different geographical region of Chhattisgarh, India

The leaves of <i>Moringa oleifera</i>, a plant well-known for its health benefits. In order to assess the effects of regional variations on the phytoconstituents found in <i>Moringa oleifera</i> Lam leaves, this work sought to develop a simple, sensitive, and accurate HPTLC method. The following solvent systems were employed to estimate the amounts of the marker medicines rutin and benzyl isothiocyanate in <i>Moringa</i> using the following methodology: Formic acid, methanol, toluene, ethyl acetate, and ethanol (4:3:2:1:0.2) v/v/v/v. Ethyl acetate: chloroform: methanol: formic acid (5:3:2:1) v/v/v/v was the solvent system used for chlorogenic acid, and toluene: ethyl acetate: methanol and formic acid (6:3:1:0.3) v/v/v/v was the solvent system best suited for the separation and measurement of β-sitosterol in leaf extract. Trial runs was held to optimise every solvent system. The Rf value for rutin is 0.21, 0.67 for β-sitosterol, 0.84 for benzoyl isothiocyanate, and 0.34 for chlorogenic acid.