Nanozyme antibiotic dressings to accelerate wound healing through anti-inflammatory and antibacterial effects

Chronic and refractory wounds pose significant clinical challenges due to persistent bacterial colonization, excessive inflammation, and impaired tissue regeneration. Herein, we report a multifunctional hydrogel, CuHP@GelMA, integrating CuHP nanozyme with GelMA, which combines cascade multienzyme-mimetic catalytic activity, antibacterial efficacy, anti-inflammatory function, and tissue regenerative potential. The CuHP nanozymes exhibit peroxidase (POD)-like activity to generate reactive oxygen species (ROS) for bacterial membrane disruption, alongside superoxide dismutase (SOD)- and catalase (CAT)-like activities to modulate oxidative stress, effectively mitigating inflammation and cellular damage. Encapsulation within the GelMA hydrogel ensures controlled release and reduced cytotoxicity, maintaining excellent biocompatibility. <i>In vitro</i>, CuHP@GelMA efficiently killed <i>Escherichia coli</i> and <i>MRSA</i> and promoted proliferation of endothelial and fibroblast cells. <i>In vivo</i>, the hydrogel accelerated wound closure, enhanced angiogenesis and collagen remodeling, and facilitated tissue regeneration without observable systemic toxicity. This work demonstrates that CuHP@GelMA serves as a versatile and clinically translatable wound-healing platform, where cascade catalytic nanozyme activity synergizes with hydrogel delivery to provide an integrated solution for infection control, inflammation modulation, and tissue repair.