Virome analysis of mango reveals mangifera indica latent virus, a benyvirus and a novel olivavirus

Mango (Mangifera indica), a globally significant fruit crop, is susceptible to diverse pathogens that threaten its productivity. High-throughput sequencing-based virome profiling and Sanger sequencing of symptomatic (mosaic, upward leaf curling with undulated margin in the young leaves) mango samples revealed the presence of a novel olivavirus (mangifera virus 1, MaV-1), and a benyvirus (mangifera indica latent virus, MiLV). Comparative genomic and phylogenetic analyses confirmed the taxonomic placement of MaV-1 within the proposed genus Olivavirus, based on conserved domains and sequence divergence in the RdRp, HSP70h, and coat protein genes. This study represents the first report of MiLV in India and a novel MaV-1 species infecting mango worldwide. Current study enhances understanding of mango viral spectrum and highlights the need for viral surveillance to address the potential threats to mango cultivation.