Anti-interference bimodule biosensors with Au/Cu <sub>2</sub> O hollow spheres for accurate detection of uric acid in human serum
The development of portable and cost-effective biosensors for uric acid (UA) monitoring is crucial for point-of-care diagnostics, yet remains challenging due to complex biological interferences. Herein, we present a flexible bimodule biosensor fabricated on a polyethylene terephthalate (PET) substrate for simultaneous UA and pH detection. The UA sensing module utilizes screen-printed electrodes (SPEs) modified with gold nanospike-decorated cuprous oxide hollow spheres (Au/Cu<sub>2</sub>O HS), enabling highly sensitive detection of UA with an ultralow detection limit of 329 nM. An independent pH module provides real-time calibration, effectively correcting signal fluctuations caused by interferents such as ascorbic acid (AA). By applying a custom calibration algorithm, the average UA detection error in phosphate-buffered saline (PBS) containing high concentrations of AA was reduced from 6.43% to 1.02%. Furthermore, the system demonstrated reliable UA detection in human serum, highlighting its strong potential for practical diagnostic applications.
Authors
- Shu Gong (ORCID: https://orcid.org/0000-0001-6904-1706)
- Ting Liu
- Zhu Xiao
- Zejun Deng
- Linyou Yi
- Jiayi Liu
- Zejun Wang
Institutions
- Beijing Normal-Hong Kong Baptist University (CN)
- Central South University (CN)
- Hong Kong Baptist University (HK)
- Second Xiangya Hospital of Central South University (CN)
Publication Details
- Journal
- Nanoscale
- Published
- 2025-12-26
- DOI
- https://doi.org/10.1039/d5nr04790k
- Primary Topic
- Electrochemical sensors and biosensors
- Type
- article
- Field-Weighted Citation Impact
- 0.00