Miniaturization of Human iPSC‐derived neuron long‐term culture into high‐throughput screening format for AD biology and therapeutics discovery

We optimized the long-term hiPSC-derived neuron culture into a 384-well HTS format for modeling AD and developed a panel of functional HTS/HCS assays for screening small molecules and biological perturbagens. The results from screening a set of well-annotated small molecules with known biological targets and activities will enable us to rapidly advance knowledge of AD targets in hiPSC-derived, AD-relevant cell types. Our HTS neuron long-term culture system will make expanded screening with large compound libraries feasible to accelerate AD drug discovery.