Cell‐Based Phenotypic and Target Engagement Assays for the Identification of Small Molecules that Enhance Microglial Phagocytosis

This combination of phenotypic and target engagement cell-based assays is widely applicable to drug discovery projects. Our studies demonstrated the feasibility of combining phenotypic assays with target engagement assays to ensure compound specificity for drug discovery projects. This strategy can be useful when specific signaling measurement is unavailable or difficult to implement for some targets.