Phytochemical Profiling and Larvicidal Activity of Ethanolic Extracts from Persea americana Mill. (Var. Lorena) Against Aedes aegypti

Dengue is a mosquito-borne viral disease transmitted by <i>Aedes aegypti</i>, the main vector in the Americas. The lack of effective antiviral treatments, limited vaccine coverage, and the increasing resistance of mosquitoes to conventional insecticides emphasize the need for alternative vector control strategies. Plant-derived larvicides represent a promising and eco-friendly approach. This study characterized the phytochemical profile of <i>Persea americana</i> Mill. (var. Lorena) and evaluated its larvicidal activity against <i>Ae. aegypti</i> (Rockefeller strain). The phytochemical profile was assessed through qualitative screening, UV-Vis spectrophotometry, and UHPLC analysis. Larvicidal activity was evaluated against third-instar larvae of <i>Ae. aegypti</i> (Rockefeller strain) and the median lethal concentration (LC₅₀) values were determined. Preliminary screening of ethanolic extracts revealed the presence of various secondary metabolites of pharmacological relevance, including alkaloids, coumarins, tannins, flavonoids, saponins, triterpenes/sterols, and quinones. UV-Vis spectra displayed distinct absorption patterns, with a prominent peak near 260 nm, consistent with the presence of aromatic compounds. UHPLC profiling revealed high chemical diversity across different plant parts, with 70, 98, 71, and 52 peaks (above 1 × 10⁵ intensity) detected in seed, flower, pulp, and leaf extracts, respectively. Larvicidal bioassays showed significant activity, particularly in the seed extract, with LC₅₀ values (µg/mL) of 3.8 (3.3-4.1) for seeds, 22.4 (21.8-23.9) for flowers, 23.0 (21.5-24.6) for pulp, and 29.7 (28.1-31.2) for leaves. This study highlights the larvicidal potential of ethanolic extracts from <i>P. americana</i> (var. Lorena), with the seed extract exhibiting the highest chemical diversity and bioactivity against <i>Ae. aegypti</i> larvae. The detection of key secondary metabolites, including flavonoids, alkaloids, and saponins, supports the development of an effective, plant-based larvicide for sustainable vector control strategies.