Analysis of Research Progress on the Chemical Constituents and Pharmacological Activities of Er-Shiwei Roudoukou Wan

Ershiwei Roudoukou Wan, a traditional Tibetan medicine, is known for its sedative and tranquilizing properties. Although considerable progress has been made in characterizing its chemical constituents and pharmacological mechanisms, a comprehensive and systematic evaluation remains limited. This study integrates bibliometric analysis with a systematic literature review to summarize current research trends, clarify the chemical basis, and assess pharmacological evidence, thereby supporting rational clinical use. Publications on Ershiwei Roudoukou Wan and its component herbs were retrieved from the Web of Science database. After screening, 7869 articles were analyzed using CiteSpace to generate knowledge maps. Research hotspots centered on pharmacological activities, chemical composition, and methodological advances. Clinically, the formula has shown efficacy in conditions including heart-gallbladder syndrome, "Ninglong disease" and gynecological disorders. Analytical methods for several marker compounds have been established, and individual herbs contain diverse bioactive constituents-predominantly terpenoids, flavonoids, polysaccharides, and tannins. Pharmacological investigations highlight cardiovascular protection, immunomodulatory and anti-inflammatory effects, anticancer activity, and neuroprotection. Despite these advances, experimental studies on the complete formulation and large-scale clinical trials remain scarce. Future research should leverage advanced analytical and pharmacological techniques to elucidate the integrated mechanisms of action and promote the modernization of Tibetan medicine.