Production of FOS (Fructooligosaccharides) with fructosyltransferase enzymes and their effect on nutrient digestion in broilers

This study aims to determine the influence of FOS on microbial populations, enzyme activity digestive tract and nutrient digestion in broilers. The research's periods were (1) FOS production; (2) the effect of FOS on intestinal bacterial, amylase and protease activity; (3) a digestibility in-vivo test. The test involved 40 broilers, which were divided into four treatments: T0: control, T1: + FOS 2 gr/kg, T2 :+ FOS 4 g/kg and T3: + FOS 6 g/kg, using a complete random design with four replicates. The incubation results in relation to fructosyltransferase enzyme for 3 hours resulted in the production of FOS 52.07%. The addition of FOS at 4 g/kg increased the population of Bifidobacterial, and decreased that of E coli bacteria (P<0.05), while at a dose of 2 g/kg it increased the activity of amylase, proteases. At the FOS dose it had a noticeable effect on improving nitrogen digestibility, calcium and phosphor (P<0.05) but no effect on the digestibility of crude fibers and metabolic energy. The study conclusion is that at 3 h of incubation were converted to more than 50% to synthesize FOS. The addition of FOS to feed is demonstrated to have a positive effect on the performance of broilers.