The effectiveness of polyherbal formulations in regenerating wounds and profiling their therapeutic properties was tracked via high-throughput methods in conjunction with In silico molecular docking

Wound healing is a complicated process that includes detailed interactions between cells and molecules. This study investigated the burn wound healing properties of a polyherbal formulation obtained from Trigonella, Lawsonia, Basella, Peristrophe and Purslane.Qualitative phytochemicals examination revealed that the extract is mostly composed of phenolic components, indicating its potential medicinal benefits. The in-vitro wound healing experiments on L929 cell lines revealed a notable increase in cell proliferation and migration after treatment with the polyherbal formulation compared with the monoherbal extract, suggesting its effectiveness in accelerating wound closure. Apigenin, coumaric acid, diosmetin, ferulic acid, lalioside, luteolin, protocatechuic acid, syringic acid and vitexin were the predominant bioactive components in the polyherbal formulation according to liquid chromatography-high-resolution mass spectrometry (LC-HRMS).Molecular docking simulations via AutoDock software revealed robust binding interactions between key components of the polyherbal formulation and wound healing receptors, such as fibroblast growth factor (4V05), epidermal growth factor receptor (6Z4B), and human matrix metalloproteinase (3SHI), indicating its possible role in wound healing mechanisms. A deeper analysis of the binding interactions showed that luteolin exhibited strong binding affinity with receptor 3SHI (-9.00 kcal/mol) due to its ability to form hydrogen bonds and hydrophobic interactions at the active site, which are critical for modulating collagenase activity during wound healing. Apigenin and vitexin also showed significant interactions with 4V05 and 6Z4B, suggesting their potential roles in promoting fibroblast proliferation and epidermal repair, respectively. These findings are supported by literature demonstrating the biological relevance of these binding interactions in wound repair pathways.Research indicates that the polyherbal formulation, which contains flavones, glycosides, and phenolic compounds, has potential as a wound healing agent because it influences important molecular targets in wound repair pathways. Additional research is needed to clarify the exact mechanisms responsible for the reported benefits and to investigate its potential therapeutic uses in clinical environments.