Functional characterization of <scp> <i>Hsk1</i> </scp> and <scp> <i>Chit1</i> </scp> genes in the virulence of <scp> <i>Metarhizium guizhouense</i> </scp> Xct1 via <scp>CRISPR</scp> ‐Cas9‐mediated gene editing
Chit1 is a critical virulence factor, influencing cell-wall integrity and insecticidal activity, while Hsk1 is essential for fungal viability. This study presents the first CRISPR-Cas9-mediated functional analysis of these genes, revealing that Chit1 overexpression enhances biocontrol efficacy against S. frugiperda. © 2025 Society of Chemical Industry.
Authors
- Chunxian Jiang (ORCID: https://orcid.org/0000-0002-8008-9396)
- Yihao Chen (ORCID: https://orcid.org/0000-0002-9956-5719)
- Jun Wei (ORCID: https://orcid.org/0009-0004-7233-3784)
- Xinyue Yang
- Qing Li
Institutions
- Sichuan Agricultural University (CN)
Publication Details
- Journal
- Pest Management Science
- Published
- 2025-12-24
- DOI
- https://doi.org/10.1002/ps.70484
- Primary Topic
- Entomopathogenic Microorganisms in Pest Control
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Modern Agricultural Technology Industry System of Shandong province