Investigation into the Anti-oxidant and Anti-Tumor Activity of the Bovine Serum Albumin Nanoparticles Containing Grape Seed Polyphenols Fabricated by Desolvation Method

Grape seed polyphenols (GPP) have been known to have various pharmaceutical activities including anti-oxidant and anti-tumor activity. However, its clinical application is hindered by low stability and bioavailability. One of the most common methods for solving these problems is to use different types of carriers. Recently, protein based drug delivery system has gained a great interest. In this study, bovine serum albumin (BSA) nanoparticles loaded GPP were manufactured by desolvation method. Several characteristics such as the average size, shape, releasing behavior and stability under UV light were tested by different techniques. Anti-oxidant activity of BSA-GPP nanoparticles were compared to free GPP. In addition, anti-tumor activity was also studied in mice model injected sarcoma-180 cells. The result showed that the shape of prepared BSA-GPP nanoparticles was spherical with the average size of 207.02nm. GPP were released by a diffusion from the nanoparticles. The nanoparticles loaded GPP showed relatively higher stability under UV condition. BSA-GPP nanoparticles maintained longer in vitro antioxidant activity than free GPP. Importantly, tumor inhibition rate of BSA-GPP nanoparticles was 84%, which was much higher than that of free GPP. In summary, BSA nanoparticles had a high potential to be used as a carrier for improving stability and increasing bioavailability of GPP.