<i>In Vitro</i> Plant Regeneration from Shoot Tip, Cotyledon and Nodal Explants of Biodiesel Plant (<i>Jatropha curcas </i>L.)

<i>In vitro</i> regeneration of <i>Jatropha curcus</i> L. was established from different explants such as shoot tips, cotyledons and nodal segments using different concentrations and combinations of plant growth regulators and additives. The nodal segments were found superior explants to shoot tips and cotyledons. The highest result obtained on multiple shoot formation from nodal explants using 2.0 mg/l BA + 2.0 mg/l kin. in MS supplemented medium, in which 70% of explants responded for multiple shooting after 30 days of inoculation period. The average number of shoots and leaves/explants were 4.2  0.64 and 2.90  0.42 in this medium 90 days after inoculation respectively. The average shoot length of 3.62  0.29 cm was also found in this same media and condition. Regenerated shoots were rooted well when transferred to of half strength of MS + 2.5 mg/l lBA, in which 50% of shoots responded after 30 days of inoculation period. The average number of roots/in-vitro raised shoots of 3.2  0.65 and the average root length of 3.5 0.29 cm were recorded in this medium after 60 days of inoculation. Plantlet regeneration was observed with low frequency in shoot tip and cotyledon explants. Most of the regenerants initially produced a clump of meristems and tended to form calli rather than multiple shoot formations.