Multilocus Sequence Typing Analysis of <i>Staphylococcus xylosus</i>

Objective: In order to explore the genetic distance and evolutionary relationship of <i>Staphylococcus xylosus</i>, and to provide a basis for rapid detection and identification of <i>Staphylococcus xylosus</i>. Method: This paper focuses on all strains of <i>Staphylococcus xylosus</i> downloaded from NCBI as the research object. Based on 8 housekeeping genes (<i>arcC</i>, <i>bglA</i>, <i>dnaJ</i>, <i>groL</i>, <i>purA</i>, <i>pyrD</i>, <i>recA</i>, and <i>tpiA</i>), a multilocus sequence typing (MLST) process was created for Multilocus sequence analysis. Result: The results indicate that the MLST method created can effectively perform typing analysis on <i>Staphylococcus xylosus</i>. Among them, the 72 strains of <i>Staphylococcus xylosus</i> included in the analysis can be classified into 53 ST types, which can be further divided into 3 subgroups. In addition, among the 8 selected housekeeping genes, <i>arcC</i> and <i>recA</i> underwent significant genetic recombination. Conclusion: In summary, the established MLST method can be used for genetic evolution analysis of <i>Staphylococcus xylosus</i>. 【目的】为了探究木糖葡萄球菌的亲缘远近和遗传进化关系，以期为木糖葡萄球菌的快速检测和鉴定提供基础。【方法】本论文以下载自NCBI的所有木糖葡萄球菌的全部菌株为研究对象，基于8个持家基因（arcC、bglA、dnaJ、groL、purA、pyrD、recA和tpiA）创建了一个多位点序列分型（Multi-locussequencetyping, MLST）流程，进行多位点序列分析。【结果】结果表明，所创建的MLST方法能较好的对木糖葡萄球菌进行分型分析，其中纳入分析的72株木糖葡萄球菌可以归类为53个ST型，进而被划分为3个亚群。另外，所选的8个持家基因中arcC和recA显著发生了基因重组。【结论】总之，建立的多位点分型方法能够用于木糖葡萄球菌的遗传进化分析。