Collaborative Study of Determination Method of Zearalenone and Deoxynivalenol in Silage by LC-MS/MS

A collaborative study was conducted by eight laboratories to validate a method for the determination of zearalenone (ZEN) and deoxynivalenol (DON) in silage using LC-MS/MS. Corn silage and whole-crop rice silage were spiked with ZEN and DON at the following levels: 0.2-2.2 mg/kg of ZEN and 1.2-14 mg/kg of DON for corn silage, and 0.48-2.6 mg/kg of ZEN and 0.8-10 mg/kg of DON for whole-crop rice silage. The resulting mean recoveries ranged from 104% to 111% for ZEN and from 97.9% to 107% for DON. The repeatability and reproducibility, expressed as relative standard deviations (RSD_r and RSD_R), were less than 6.1% and 8.8% for ZEN, and less than 8.3% and 19% for DON, respectively. The HorRat values were less than 0.53 for ZEN and less than 1.2 for DON. These results demonstrated that the method is suitable for the inspection of ZEN and DON in silage.