Apoptosis induction by Cassia angustifolia leaf extract in breast cancer cell line and intracellular expression of inflammatory cytokine

Cassia angustifolia is a famous and important medicinal plant around the world. It is used for the treatment of a lot of diseases to reduce inflammation in the skin and other parts of the body. Breast cancer has been distributed at a high rate in recent years among women and be most threatening to life. In this study the activity of Cassia angustifolia leaf extract was investigated as anticancer for breast cancer and immune response enhancement. WRL68, MCF7 and MDA-MB-231 cell line were treated with different doses of C. angustifolia leaf extract. Macrophage RAW 246.7 cell line was treated with C. angustifolia ethanol leaf extract for enhanced IL8, INF gamma and IL6 intracellular expression. Results showed that C. angustifolia ethanol leaf extract showed no toxic effect and did not affect the viability of WRL-68 liver cell line, while inhibiting the viability of breast cancer cell line MDA-MB-231 and MCF7 cell line a dose-dependent manner as presented. Also, influence on the intracellular expression of inflammatory cytokines as IL8, INF- gamma and IL6 were revealed enhanced significant the expression these cytokines within treated RAW cells with C. angustifolia leaf extract. To conclude, C. angustifolia ethanol leaf extract has anti-proliferation agent and enhancement of immune response by enhancing the intracellular expression of IL8, IFN-gamma and IL6 cytokines in the treated macrophage RAW cell line.